Characterizing the Syphilis-Causing Treponema pallidum ssp pallidum Proteome Using Complementary Mass Spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00088426" target="_blank" >RIV/00216224:14110/16:00088426 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pntd.0004988" target="_blank" >http://dx.doi.org/10.1371/journal.pntd.0004988</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pntd.0004988" target="_blank" >10.1371/journal.pntd.0004988</a>
Alternative languages
Result language
angličtina
Original language name
Characterizing the Syphilis-Causing Treponema pallidum ssp pallidum Proteome Using Complementary Mass Spectrometry
Original language description
Background The spirochete bacterium Treponema pallidum ssp. pallidum is the etiological agent of syphilis, a chronic multistage disease. Little is known about the global T. pallidum proteome, therefore mass spectrometry studies are needed to bring insights into pathogenicity and protein expression profiles during infection. Methodology/Principal Findings To better understand the T. pallidum proteome profile during infection, we studied T. pallidum ssp. pallidum DAL-1 strain bacteria isolated from rabbits using complementary mass spectrometry techniques, including multidimensional peptide separation and protein identification via matrix-assisted laser desorption ionization-time of flight (MALDI-TOF/TOF) and electrospray ionization (ESI-LTQ-Orbitrap) tandem mass spectrometry. A total of 6033 peptides were detected, corresponding to 557 unique T. pallidum proteins at a high level of confidence, representing 54% of the predicted proteome. A previous gel-based T.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FN - Epidemiology, infection diseases and clinical immunology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS neglected tropical diseases
ISSN
1935-2735
e-ISSN
—
Volume of the periodical
10
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
29
Pages from-to
1-29
UT code for WoS article
000385627900043
EID of the result in the Scopus database
—