Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00089182" target="_blank" >RIV/00216224:14110/16:00089182 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/boc.201500047" target="_blank" >http://dx.doi.org/10.1111/boc.201500047</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/boc.201500047" target="_blank" >10.1111/boc.201500047</a>
Alternative languages
Result language
angličtina
Original language name
Introduction of macarpine as a novel cell-permeant DNA dye for live cell imaging and flow cytometry sorting
Original language description
Macarpine (MA) is a quaternary benzophenanthridine plant alkaloid isolated from Macleaya microcarpa or Stylophorum lasiocarpum. Benzophenanthridine alkaloids are interesting natural products that display antiproliferative, antimicrobial, antifungal and anti-inflammatory activities, and also fluorescence properties. In a previous study, we demonstrated that thanks to its ability to interact with DNA and its spectral properties MA could be used as a supravital DNA probe for fluorescence microscopy and flow cytometry including analyses of the cell cycle. In this study, we evaluated the suitability of MA as a DNA dye for time-lapse microscopy and flow-cytometric cell sorting.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LH12176" target="_blank" >LH12176: Benzophenanthridine alkaloids - study of their effects on cellular and molecular level</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biology of the Cell
ISSN
0248-4900
e-ISSN
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Volume of the periodical
108
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
1-18
UT code for WoS article
000369960200001
EID of the result in the Scopus database
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