PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F17%3A00094599" target="_blank" >RIV/00216224:14110/17:00094599 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1091/mbc.E16-09-0686" target="_blank" >http://dx.doi.org/10.1091/mbc.E16-09-0686</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1091/mbc.E16-09-0686" target="_blank" >10.1091/mbc.E16-09-0686</a>
Alternative languages
Result language
angličtina
Original language name
PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis
Original language description
In mammals, meiotic recombination occurs at 1-2 kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We now show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and co-immunoprecipitation from mouse spermatocytes, we identified four proteins that directly interact with PRDM9's KRAB domain, namely CXXC1, EWSR1, EHMT2, and CDYL. These proteins are co-expressed in spermatocytes at the early stages of meiotic prophase I, the limited period when PRDM9 is expressed. We also detected association of PRDM9-bound complexes with the meiotic cohesin REC8 and the synaptonemal complex proteins SYCP3 and SYCP1. Our results suggest a model in which PRDM9-bound hotspot DNA is brought to the chromosomal axis by the action of these proteins, ensuring the proper chromatin and spatial environment for subsequent recombination events.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Biology of the Cell
ISSN
1059-1524
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
488-499
UT code for WoS article
000394268700014
EID of the result in the Scopus database
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