The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F17%3A00095676" target="_blank" >RIV/00216224:14110/17:00095676 - isvavai.cz</a>
Alternative codes found
RIV/65269705:_____/17:00067525
Result on the web
<a href="http://dx.doi.org/10.1111/and.12702" target="_blank" >http://dx.doi.org/10.1111/and.12702</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/and.12702" target="_blank" >10.1111/and.12702</a>
Alternative languages
Result language
angličtina
Original language name
The intracellular concentration of homocysteine and related thiols is negatively correlated to sperm quality after highly effective method of sperm lysis
Original language description
The aims of this study were to determine concentrations of total homocysteine, cysteine, cysteinylglycine and glutathione in spermatozoa, seminal fluid and blood plasma and to analyse their relationships with sperm parameters. For this reason, a new highly effective method of spermatozoa lysis was developed, using methanol, freezing and subsequent thawing in ultrasonic bath. An HPLC-FD assay was conducted on thiols concentrations in lysed spermatozoa, seminal fluid and blood plasma. Concentrations of thiols in spermatozoa were significantly lower in men with normozoospermia than in samples with pathological semen parameters. Statistical analysis found significant correlations between thiol concentrations in spermatozoa and semen parameters, while the same analysis with thiol concentrations in seminal fluid was substantially less powerful. Only cysteinylglycine concentrations in seminal fluid significantly correlated with pathological semen parameters. No significant differences or correlations were found with blood plasma concentrations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
<a href="/en/project/NS9661" target="_blank" >NS9661: Impact of homocysteine metabolism on human infertility</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ANDROLOGIA
ISSN
0303-4569
e-ISSN
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Volume of the periodical
49
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
1-5
UT code for WoS article
000408626900011
EID of the result in the Scopus database
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