Upregulation of receptors for mitochondrial damage associated molecular patterns in the dorsal root ganglia of experimental neuropathic pain models based on sciatic nerve injury

Result code in IS VaVaI

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Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Upregulation of receptors for mitochondrial damage associated molecular patterns in the dorsal root ganglia of experimental neuropathic pain models based on sciatic nerve injury

Original language description

Upregulation of receptors for mitochondrial damage associated molecular patterns in the dorsal root ganglia of experimental neuropathic pain models based on sciatic nerve injury Dubový P., Klusáková I., Joukal M., Hradilová-Svíženská I. Department of Anatomy, Cellular and Molecular Neurobiology Group, Faculty of Medicine, Masaryk University, Kamenice 3, 62500 Brno, Czech Republic Most frequently used experimental models of neuropathic pain (NPP) are based on chronic constriction injury (CCI) or spared nerve injury (SNI) of the rat sciatic nerve. Disintegration of mitochondria including release of mitochondrial proteins and their DNAs (mtDNAs) is present in Wallerian degeneration (WD) distal to nerve injury. N-formyl peptides released by ruptured mitochondria are ligands of the formyl-peptide receptor 2 (FPR2) and mtDNA sequences containing unmethylated CpG dinucleotides activate Toll-like receptor 9 (TLR9). We studied cellular distribution of FPR2 and TLR9 by immunohistochemical detection in the dorsal root ganglia (DRG) associated (L4-L5 segments) and non-associated (C6-C8 segments) with the sciatic nerve injury of both CCI and SNI models. An induction of NPP was tested by measurement of withdrawal thresholds for mechanical hypersensitivity and latencies for thermal hypersensitivity. We found a low intensity of FPR2 and TLR9 immunofluorescence in DRG neurons of all populations. Unilateral CCI and SNI of the sciatic nerve induced mechanical and thermal hypersensitivity in operated hind paws. However, increased FPR2 and TLR9 immunofluorescence intensity was measured bilaterally not only in DRG of L4-L5 segments, but also in remote C6-C8 DRG. Increased expression of FPR2 and TLR9 predominantly in neuronal bodies corresponds with bilateral upregulation of inflammatory mediators (1, 2). Our results suggest that inflammatory reactions in DRG might be mediated by receptors for mitochondrial damage associated molecular patterns produced by WD. In addition, the present results provide further evidence that inflammatory reaction after unilateral sciatic nerve injury is not limited to associated DRG, but can spread alongside of neuroaxis. A contribution of induced inflammation in non-impaired neurons of remote DRG to NPP remains to be elucidated. It seems that Surviving primary injury to next impairment it 1. Dubový et al., Histochem. Cell Biol. 133, 323-337 (2010). 2. Dubový et al., J. Neuroinflamm, 10, 55 (2013). Acknowledgements. The work was supported by grant 16-08508S of The Czech Science Foundation (GACR). We thank Bc. Jitka Mikulášková, Ms. Dana Kutějová and Ms. Marta Lněníčková for their skillful technical assistance.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

<a href="/en/project/GA16-08508S" target="_blank" >GA16-08508S: Cytokine/chemokine-mediated augmentation of intrinsic regeneration program and its activation in the neurons without connection to injured nerve</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů