Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00101318" target="_blank" >RIV/00216224:14110/18:00101318 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/18:00495178 RIV/00159816:_____/18:00069302 RIV/00216208:11150/18:10376156 RIV/61989592:15110/18:73587816

Result on the web

<a href="http://dx.doi.org/10.1007/s10815-018-1230-5" target="_blank" >http://dx.doi.org/10.1007/s10815-018-1230-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10815-018-1230-5" target="_blank" >10.1007/s10815-018-1230-5</a>

Result language

angličtina

Original language name

Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes

Original language description

The purpose of the study was to determine whether the GDF-15 is present in follicular fluid; to evaluate if there is a relation between follicular and serum levels of GDF-15 and fertility status of study subjects; and to test whether granulosa cells, oocytes, or both produce GDF-15. This study used follicular fluid (FF, serum, and oocytes obtained under informed consent from women undergoing oocyte retrieval for in vitro fertilization. It also used ovaries from deceased preterm newborns. Collection of FF and blood at the time of oocyte retrieval, ELISA and western blot were performed to determine levels and forms of GDF-15. Concentrations of GDF-15 in FF and serum, its expression in ovarian tissue, and secretion from granulosa cells were analyzed. GDF-15 concentration in FF ranged from 35 to 572 ng/ml, as determined by ELISA. Western blot analysis revealed the GDF-15 pro-dimer only in FF. Both normal healthy and cancerous granulosa cells secreted GDF-15 into culture media. Primary oocytes displayed cytoplasmic GDF-15 positivity in immunostained newborn ovaries, and its expression was also observed in fully grown human oocytes. To the best of our knowledge, this is the first documentation of cytokine GDF-15 presence in follicular fluid. Its concentration was not associated with donor/patient fertility status. Our data also show that GDF-15 is expressed and inducible in both normal healthy and cancerous granulosa cells, as well as in oocytes.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Assisted Reproduction and Genetics

ISSN

1058-0468

e-ISSN

1573-7330

Volume of the periodical

35

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

1407-1417

UT code for WoS article

000441294700009

EID of the result in the Scopus database

2-s2.0-85048471002