Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00102906" target="_blank" >RIV/00216224:14110/18:00102906 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jchromb.2018.05.037" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2018.05.037</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jchromb.2018.05.037" target="_blank" >10.1016/j.jchromb.2018.05.037</a>
Alternative languages
Result language
angličtina
Original language name
Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography
Original language description
Liquid chromatography with fluorescence detection has been used in analyses demanding high sensitivity and selectivity. As majority systems rely on reversed-phase columns with water being the main component of the mobile phase, fluorescent compounds with emission maxima higher than 500 nm might be dynamically quenched. A simple replacement of H2O with D2O enhanced the sensitivity for selected compounds by 10–200 per cent. Affected compounds included an anti-cancer drug doxorubicin, a luminescent probe fluorescein, and naturally occurring forms of vitamin B2. Similar levels of enhancement were obtained by fluorescence spectrometry. Such simple yet effective approach may greatly improve HPLC analyses coupled to fluorescence detection.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN
1570-0232
e-ISSN
1873-376X
Volume of the periodical
1092
Issue of the periodical within the volume
AUG 15 2018
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
7-14
UT code for WoS article
000439403100002
EID of the result in the Scopus database
2-s2.0-85047786978