Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00104105" target="_blank" >RIV/00216224:14110/18:00104105 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1371/journal.pntd.0006396" target="_blank" >http://dx.doi.org/10.1371/journal.pntd.0006396</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pntd.0006396" target="_blank" >10.1371/journal.pntd.0006396</a>

Result language

angličtina

Original language name

Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies

Original language description

We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are known to infect humans and nonhuman primates (NHPs). Four TPA, six human, and two NHP TPE strains, as well as two human TEN strains were used to establish and validate the LAMP assays. All three LAMP assays were highly specific for the target DNA. Amplification was rapid (5-15 min) and within a range of 10E+6 to 10E+2 of target DNA molecules. Performance in NHP clinical samples was similar to the one seen in human TPE strains. The newly designed LAMP assays provide proof of concept for a diagnostic tool that enhances yaws clinical diagnosis. It is highly specific for the target DNA and does not require expensive laboratory equipment. Test results can potentially be interpreted with the naked eye, which makes it suitable for the use in remote clinical settings.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30309 - Tropical medicine

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS neglected tropical diseases

ISSN

1935-2735

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

1-14

UT code for WoS article

000433487700036

EID of the result in the Scopus database

2-s2.0-85045024989