Metal-induced oxidative stress in terrestrial macrolichens
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00104164" target="_blank" >RIV/00216224:14110/18:00104164 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.chemosphere.2018.03.112" target="_blank" >http://dx.doi.org/10.1016/j.chemosphere.2018.03.112</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chemosphere.2018.03.112" target="_blank" >10.1016/j.chemosphere.2018.03.112</a>
Alternative languages
Result language
angličtina
Original language name
Metal-induced oxidative stress in terrestrial macrolichens
Original language description
Short-term (24 h) responses of Cladonia arbuscula subsp. mitis and Cladonia furcate to copper (CuII) or chromium (CrIII) excess (10 or 100 mu M) were compared. C arbuscula accumulated more Cu and Cr at higher metal doses but both species revealed depletion of K and/or Ca amount. Not only Cu but also Cr typically elevated reactive oxygen species (ROS) formation (fluorescence microscopy detection of total ROS and hydrogen peroxide) and depleted nitric oxide (NO) signal, with Cu showing more negative impact on lipid peroxidation (BODIPY 581/591 C11 staining reagent). Metals and staining reagents also affected anatomical responses and photobiont/mycobiont visibility. Principally different impact of Cu and Cr was observed at antioxidative metabolites level, indicating various ways of metal-induced ROS removal and/or metal chelation: Cu strongly depleted glutathione (GSH) and stimulated phytochelatin 2 (PC2) content while ascorbic acid accumulation was depleted by Cu and stimulated by Cr. Subsequent experiment with GSH biosynthetic inhibitor (buthionine sulfoximine, BSO) revealed that 48 h of exposure is needed to deplete GSH and BSO-induced depletion of GSH and PC2 amounts under Cu or Cr excess elevated ROS but depleted NO. These data suggest close relations between thiols, NO and appearance of oxidative stress (ROS generation) under metallic stress also in lichens. (C) 2018 Elsevier Ltd. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemosphere
ISSN
0045-6535
e-ISSN
1879-1298
Volume of the periodical
203
Issue of the periodical within the volume
JUL 2018
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
402-409
UT code for WoS article
000432235000046
EID of the result in the Scopus database
2-s2.0-85047487633