Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F19%3A00112504" target="_blank" >RIV/00216224:14110/19:00112504 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1177/0956462419859764" target="_blank" >http://dx.doi.org/10.1177/0956462419859764</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1177/0956462419859764" target="_blank" >10.1177/0956462419859764</a>
Alternative languages
Result language
angličtina
Original language name
Accuracy of PCR and serological testing for the diagnosis of primary syphilis: Both tests are necessary
Original language description
Syphilis, caused by the spirochete Treponema pallidum subspecies pallidum, is a rising global public health concern and laboratory diagnostics remain challenging. Especially during early disease, rapid and accurate diagnosis is crucial to ensure patients and their contacts receive timely treatment to eradicate infection and prevent further transmission. In this prospective observational study, we evaluated the performance of polymerase chain reaction (PCR) and serological testing for the diagnosis of primary syphilis by evaluating anogenital swabs and sera from 178 Cuban patients presenting with ulcers. Three different PCR assays were evaluated targeting polA, tpp47 and 16S rDNA loci. Sera were evaluated with venereal disease research laboratory (VDRL) and T. pallidum hemagglutination (TPHA) assays. Assuming both methods were confirmatory, our data showed that PCR and serology did not correlate well (agreement = 52.3%, kappa 0.0512, 95% CI -0.0928-0.1951, p = 0.496). The sensitivities, specificities, positive and negative predictive values of the PCR assays were 76.1%, 100%, 100% and 57.9%, respectively, while the values for serology were 62.5%, 100%, 100% and 45.2%, respectively. The combination of PCR and serology can offer valuable information for the diagnosis of syphilis in patients presenting with anogenital ulceration avoiding further clinical complications and disease transmission.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
INTERNATIONAL JOURNAL OF STD & AIDS
ISSN
0956-4624
e-ISSN
1758-1052
Volume of the periodical
30
Issue of the periodical within the volume
11
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
1087-1094
UT code for WoS article
000490799700001
EID of the result in the Scopus database
2-s2.0-85073943631