RAD51-dependent recruitment of TERRA lncRNA to telomeres through R-loops

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F20%3A00114561" target="_blank" >RIV/00216224:14110/20:00114561 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/20:00074098

Result on the web

<a href="https://www.nature.com/articles/s41586-020-2815-6" target="_blank" >https://www.nature.com/articles/s41586-020-2815-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41586-020-2815-6" target="_blank" >10.1038/s41586-020-2815-6</a>

Result language

angličtina

Original language name

RAD51-dependent recruitment of TERRA lncRNA to telomeres through R-loops

Original language description

Telomeres- repeated, noncoding nucleotide motifs and associated proteins that are found at the ends of eukaryotic chromosomes—mediate genome stability and determine cellular lifespan. Telomeric-repeat-containing RNA (TERRA) is a class of long noncoding RNAs (lncRNAs) that are transcribed from chromosome ends; these RNAs in turn regulate telomeric chromatin structure and telomere maintenance through the telomere-extending enzyme telomerase and homology-directed DNA repair. The mechanisms by which TERRA is recruited to chromosome ends remain poorly defined. Here we develop a reporter system with which to dissect the underlying mechanisms, and show that the UUAGGG repeats of TERRA are both necessary and sufficient to target TERRA to chromosome ends. TERRA preferentially associates with short telomeres through the formation of telomeric DNA–RNA hybrid (R-loop) structures that can form in trans. Telomere association and R-loop formation trigger telomere fragility and are promoted by the recombinase RAD51 and its interacting partner BRCA2, but counteracted by the RNA-surveillance factors RNaseH1 and TRF1. RAD51 physically interacts with TERRA and catalyses R-loop formation with TERRA in vitro, suggesting a direct involvement of this DNA recombinase in the recruitment of TERRA by strand invasion. Together, our findings reveal a RAD51-dependent pathway that governs TERRA-mediated R-loop formation after transcription, providing a mechanism for the recruitment of lncRNAs to new loci in trans.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature

ISSN

0028-0836

e-ISSN

1476-4687

Volume of the periodical

587

Issue of the periodical within the volume

7833

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

303-308

UT code for WoS article

000624288600001

EID of the result in the Scopus database

2-s2.0-85092497350