Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F20%3A00115971" target="_blank" >RIV/00216224:14110/20:00115971 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s00709-019-01448-1" target="_blank" >https://link.springer.com/article/10.1007/s00709-019-01448-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00709-019-01448-1" target="_blank" >10.1007/s00709-019-01448-1</a>
Alternative languages
Result language
angličtina
Original language name
Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum
Original language description
Impact of various nitrate concentrations (14.12 mM, 3.53 mM, no nitrate) or ammonium presence (14.12 mM) on physiological and metabolic changes in Hypericum perforatum after 14 days of cultivation was monitored. Nitrate deficiency suppressed growth of shoots but stimulated root growth while ammonium suppressed root growth: concomitant changes of ascorbic acid and glutathione supported these growth changes, e.g., unaltered level in roots under nitrate deficiency but depleted in ammonium treatment. Soluble proteins and water content were more suppressed by nitrate deficiency but total ROS, nitric oxide formation, and antioxidative enzyme activities (APX and SOD) indicate higher sensitivity of plants to ammonium. Though both extreme treatments (NO3- deficiency or ammonium) stimulated accumulation of total soluble phenols and affected PAL activity (in comparison with full or 1/4x nitrate dose), major phenols (chlorogenic acid and three flavonoids) were elevated mainly by NO3- deficiency. At the level of specific metabolites, NO3- deficiency had stimulatory impact on pseudohypericin (but not hypericin) content while hyperforin decreased. Expression of earlier putative gene of hypericin biosynthesis (hyp-1) showed rather partial correlation with pseudohypericin amount. Data indicate that depletion of NO3- is useful to obtain Hypericum plants with higher amount of health-positive secondary metabolites.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protoplasma
ISSN
0033-183X
e-ISSN
1615-6102
Volume of the periodical
257
Issue of the periodical within the volume
2
Country of publishing house
AT - AUSTRIA
Number of pages
9
Pages from-to
439-447
UT code for WoS article
000519379600009
EID of the result in the Scopus database
2-s2.0-85075466453