New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F20%3A00116243" target="_blank" >RIV/00216224:14110/20:00116243 - isvavai.cz</a>

Alternative codes found

RIV/65269705:_____/20:00072696

Result on the web

<a href="https://www.spandidos-publications.com/10.3892/mmr.2020.10963" target="_blank" >https://www.spandidos-publications.com/10.3892/mmr.2020.10963</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/mmr.2020.10963" target="_blank" >10.3892/mmr.2020.10963</a>

Result language

angličtina

Original language name

New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation

Original language description

Oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well-known, its exact molecular mechanisms remain widely undiscovered. Oocyte growth, differentiation and maturation are therefore the subject of scientific debate. Precious literature has indicated that the oocyte itself serves a regulatory role in the mechanisms underlying these processes. Hence, the present study performed expression microarrays to analyze the complete transcriptome of porcine oocytes during their in vitro maturation (IVM). Pig material was used for experimentation, as it possesses similarities to the reproductive processes and general genetic proximities of Sus scrofa to human. Oocytes, isolated from the ovaries of slaughtered animals were assessed via the Brilliant Cresyl Blue test and directed to IVM. A number of oocytes were left to be analyzed as the 'before IVM' group. Oocyte mRNA was isolated and used for microarray analysis, which was subsequently validated via RT-qPCR. The current study particularly focused on genes belonging to 'positive regulation of transcription, DNA-dependent', 'positive regulation of gene expression', 'positive regulation of macromolecule metabolic process' and 'positive regulation of transcription from RNA polymerase II promoter' ontologies. FOS, VEGFA, ESR1, AR, CCND2, EGR2, ENDRA, GJA1, INHBA, IHH, INSR, APP, WWTR1, SMARCA1, NFAT5, SMAD4, MAP3K1, EGR1, RORA, ECE1, NR5A1, KIT, IKZF2, MEF2C, SH3D19, MITF and PSMB4 were all determined to be significantly altered (fold change, &gt;|2|; P&lt;0.05) among these groups, with their downregulation being observed after IVM. Genes with the most altered expressions were analyzed and considered to be potential markers of maturation associated with transcription regulation and macromolecule metabolism process.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Medicine Reports

ISSN

1791-2997

e-ISSN

1791-3004

Volume of the periodical

21

Issue of the periodical within the volume

3

Country of publishing house

GR - GREECE

Number of pages

15

Pages from-to

1537-1551

UT code for WoS article

000515676300061

EID of the result in the Scopus database

2-s2.0-85078719696