High-Resolution 3D Reconstruction of Human Oocytes Using Focused Ion Beam Scanning Electron Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F21%3A00119417" target="_blank" >RIV/00216224:14110/21:00119417 - isvavai.cz</a>
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fcell.2021.755740/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcell.2021.755740/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fcell.2021.755740" target="_blank" >10.3389/fcell.2021.755740</a>
Alternative languages
Result language
angličtina
Original language name
High-Resolution 3D Reconstruction of Human Oocytes Using Focused Ion Beam Scanning Electron Microscopy
Original language description
The egg plays a pivotal role in the reproduction of our species. Nevertheless, its fundamental biology remains elusive. Transmission electron microscopy is traditionally used to inspect the ultrastructure of female gametes. However, two-dimensional micrographs contain only fragmentary information about the spatial organization of the complex oocyte cytoplasm. Here, we employed the Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) to explore human oocyte intracellular morphology in three dimensions (3D). Volume reconstruction of generated image stacks provided an unprecedented view of ooplasmic architecture. Organelle distribution patterns observed in nine donor oocytes, representing three maturational stages, documented structural changes underlying the process by which the egg acquires developmental competence. 3D image segmentation was performed to extract information about distinct organelle populations, and the following quantitative analysis revealed that the mitochondrion occupies similar to 4.26% of the maturing oocyte cytoplasm. In summary, this proof-of-concept study demonstrates the potential of large volume electron microscopy to study rare samples of delicate female gametes and paves the way for applying the FIB-SEM technique in human oocyte research.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Cell and Developmental Biology
ISSN
2296-634X
e-ISSN
2296-634X
Volume of the periodical
9
Issue of the periodical within the volume
November 2021
Country of publishing house
CH - SWITZERLAND
Number of pages
8
Pages from-to
1-8
UT code for WoS article
000719669700001
EID of the result in the Scopus database
2-s2.0-85119349234