Live birth achieved despite the absence of ejaculated spermatozoa and mature oocytes retrieved: a case report

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F21%3A00121111" target="_blank" >RIV/00216224:14110/21:00121111 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s10815-021-02070-y" target="_blank" >https://link.springer.com/article/10.1007/s10815-021-02070-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10815-021-02070-y" target="_blank" >10.1007/s10815-021-02070-y</a>

Result language

angličtina

Original language name

Live birth achieved despite the absence of ejaculated spermatozoa and mature oocytes retrieved: a case report

Original language description

The most common reason for in vitro fertilization (IVF) cycle cancelation is a lack of quality gametes available for intracytoplasmic sperm injection (ICSI). Here we present the successful fertility treatment of the couple affected by obstructive azoospermia combined with suboptimal response to controlled ovarian stimulation. Since the conventional approach appeared ineffective to overcome both partners| specific problems, the targeted interventions, namely, (1) pharmacological enhancement of sperm motility and (2) polarized light microscopy (PLM)-guided optimization of ICSI time, were applied to rescue the cycle with only immature oocytes and immotile testicular sperm retrieved. The treatment with theophylline aided the selection of viable spermatozoa derived from cryopreserved testicular tissue. When the traditional stimulation protocol failed to produce mature eggs, non-invasive spindle imaging was employed to adjust the sperm injection time to the maturational stage of oocytes extruding a polar body in vitro. The fertilization of 12 late-maturing oocytes yielded 5 zygotes, which all developed into blastocysts. One embryo was transferred into the uterus on day 5 post-fertilization, and another 3 good quality blastocysts were vitrified for later use. The pregnancy resulted in a full-term delivery of a healthy child. This case demonstrates that the individualization beyond the standard IVF protocols should be considered to maximize the chance of poor-prognosis patients to achieve pregnancy with their own gametes.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10604 - Reproductive biology (medical aspects to be 3)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Assisted Reproduction and Genetics

ISSN

1058-0468

e-ISSN

1573-7330

Volume of the periodical

38

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

925-929

UT code for WoS article

000609041700001

EID of the result in the Scopus database

2-s2.0-85099589759