Rapid Isolation of Single Cells from Mouse and Human Teeth
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F21%3A00124075" target="_blank" >RIV/00216224:14110/21:00124075 - isvavai.cz</a>
Result on the web
<a href="https://www.jove.com/t/63043/rapid-isolation-of-single-cells-from-mouse-and-human-teeth" target="_blank" >https://www.jove.com/t/63043/rapid-isolation-of-single-cells-from-mouse-and-human-teeth</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/63043" target="_blank" >10.3791/63043</a>
Alternative languages
Result language
angličtina
Original language name
Rapid Isolation of Single Cells from Mouse and Human Teeth
Original language description
Mouse and human teeth represent challenging organs for quick and efficient cell isolation for single-cell transcriptomic or other applications. The dental pulp tissue, rich in the extracellular matrix, requires a long and tedious dissociation process that is typically beyond the reasonable time for single-cell transcriptomics. For avoiding artificial changes in gene expression, the time elapsed from euthanizing an animal until the analysis of single cells needs to be minimized. This work presents a fast protocol enabling to obtain single-cell suspension from mouse and human teeth in an excellent quality suitable for scRNA-seq (single-cell RNA-sequencing). This protocol is based on accelerated tissue isolation steps, enzymatic digestion, and subsequent preparation of final single-cell suspension. This enables fast and gentle processing of tissues and allows using more animal or human samples for obtaining cell suspensions with high viability and minimal transcriptional changes. It is anticipated that this protocol might guide researchers interested in performing the scRNA-seq not only on the mouse or human teeth but also on other extracellular matrix-rich tissues, including cartilage, dense connective tissue, and dermis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Jove-Journal of Visualized Experiments
ISSN
1940-087X
e-ISSN
—
Volume of the periodical
2021
Issue of the periodical within the volume
176
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
1-12
UT code for WoS article
000715060500029
EID of the result in the Scopus database
2-s2.0-85120778000