Schwann cell precursors represent a neural crest-like state with biased multipotency
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F22%3A00127652" target="_blank" >RIV/00216224:14110/22:00127652 - isvavai.cz</a>
Result on the web
<a href="https://www.embopress.org/doi/full/10.15252/embj.2021108780" target="_blank" >https://www.embopress.org/doi/full/10.15252/embj.2021108780</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15252/embj.2021108780" target="_blank" >10.15252/embj.2021108780</a>
Alternative languages
Result language
angličtina
Original language name
Schwann cell precursors represent a neural crest-like state with biased multipotency
Original language description
Schwann cell precursors (SCPs) are nerve-associated progenitors that can generate myelinating and non-myelinating Schwann cells but also are multipotent like the neural crest cells from which they originate. SCPs are omnipresent along outgrowing peripheral nerves throughout the body of vertebrate embryos. By using single-cell transcriptomics to generate a gene expression atlas of the entire neural crest lineage, we show that early SCPs and late migratory crest cells have similar transcriptional profiles characterised by a multipotent "hub" state containing cells biased towards traditional neural crest fates. SCPs keep diverging from the neural crest after being primed towards terminal Schwann cells and other fates, with different subtypes residing in distinct anatomical locations. Functional experiments using CRISPR-Cas9 loss-of-function further show that knockout of the common "hub" gene Sox8 causes defects in neural crest-derived cells along peripheral nerves by facilitating differentiation of SCPs towards sympathoadrenal fates. Finally, specific tumour populations found in melanoma, neurofibroma and neuroblastoma map to different stages of SCP/Schwann cell development. Overall, SCPs resemble migrating neural crest cells that maintain multipotency and become transcriptionally primed towards distinct lineages.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
The Embo Journal
ISSN
0261-4189
e-ISSN
1460-2075
Volume of the periodical
41
Issue of the periodical within the volume
17
Country of publishing house
US - UNITED STATES
Number of pages
28
Pages from-to
1-28
UT code for WoS article
000822696300001
EID of the result in the Scopus database
2-s2.0-85133716524