Cryptic inhibitory regions nearby activation domains
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F22%3A00129669" target="_blank" >RIV/00216224:14110/22:00129669 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S030090842200116X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S030090842200116X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.biochi.2022.05.004" target="_blank" >10.1016/j.biochi.2022.05.004</a>
Alternative languages
Result language
angličtina
Original language name
Cryptic inhibitory regions nearby activation domains
Original language description
Previously, the Nine amino acid TransActivation Domain (9aaTAD) was identified in the Gal4 region 862–870 (DDVYNYLFD). Here, we identified 9aaTADs in the distal Gal4 orthologs by our prediction algorithm and found their conservation in the family. The 9aaTAD function as strong activators was demonstrated. We identified adjacent Gal4 region 871–811 (DEDTPPNPKKE) as a natural 9aaTAD inhibitory domain located at the extreme Gal4 terminus. Moreover, we identified conserved Gal4 region 172–185 (FDWSEEDDMSDGLP), which was capable to reverse the 9aaTAD inhibition. In conclusion, our results uncover the existence of the cryptic inhibitory domains, which need to be carefully implemented in all functional studies with transcription factors to avoid incorrect conclusions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
<a href="/en/project/NV19-05-00410" target="_blank" >NV19-05-00410: Role of cytotoxic gamma-delta T cells implicated in therapeutic resistence and tumour recurrence in Glioblastoma Multiforme</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochimie
ISSN
0300-9084
e-ISSN
1638-6183
Volume of the periodical
200
Issue of the periodical within the volume
September 2022
Country of publishing house
FR - FRANCE
Number of pages
8
Pages from-to
19-26
UT code for WoS article
000806357600003
EID of the result in the Scopus database
2-s2.0-85130334483