Assay of colistin A, B and colistin methanesulfonate in human plasma by LC-MS/MS and short-term plasma stability
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F23%3A00132006" target="_blank" >RIV/00216224:14110/23:00132006 - isvavai.cz</a>
Result on the web
<a href="https://www.klinickafarmakologie.cz/pdfs/far/2023/03/02.pdf" target="_blank" >https://www.klinickafarmakologie.cz/pdfs/far/2023/03/02.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.36290/far.2023.016" target="_blank" >10.36290/far.2023.016</a>
Alternative languages
Result language
angličtina
Original language name
Assay of colistin A, B and colistin methanesulfonate in human plasma by LC-MS/MS and short-term plasma stability
Original language description
A simple liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for the determination of colistin A and colistin B in human plasma was developed and validated. Plasma extraction was performed using Oasis HLB 1 ml cartridges, analysis was performed using Arion® Polar C18 (250×4,6mm; 5mm) column at 35°C. Mobile phases consisted of water containing 0,1% formic acid and methanol containing 0,1% formic acid (40:60, v/v) delivered at a flow rate of 0,8 ml/minute. Eluent was detected in the positive ion mode using electrospray ionization at the following transitions of mass to charge (m/z): colistin A 585,55 → 101,05; colistin B 578,5 → 101,15; and IS 602,4 → 101,1; 120,15; 86,15. Short-term stability tests of colistin and CMS were performed, at room temperature and 37°C, where the stability of both components decreases with increasing temperature. The presented paper is part of the Pharmacokinetics of Colistin in Critically Ill Patients With Extracorporeal Membrane Oxygenation (COL-ECMO2022) study in which further results will be presented.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
<a href="/en/project/LM2023049" target="_blank" >LM2023049: Czech National Node of the European Clinical Research Infrastructures Network</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Klinicka Farmakologie a Farmacie
ISSN
1212-7973
e-ISSN
1803-5353
Volume of the periodical
37
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
4
Pages from-to
89-92
UT code for WoS article
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EID of the result in the Scopus database
999