Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F23%3A00133308" target="_blank" >RIV/00216224:14110/23:00133308 - isvavai.cz</a>

Result on the web

<a href="https://www.futuremedicine.com/doi/10.2217/epi-2023-0267" target="_blank" >https://www.futuremedicine.com/doi/10.2217/epi-2023-0267</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2217/epi-2023-0267" target="_blank" >10.2217/epi-2023-0267</a>

Result language

angličtina

Original language name

Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming

Original language description

Aim:Human induced pluripotent stem cells (iPSCs) are inefficiently derived from somatic cells by overexpression of defined transcription factors. Overexpression of H2A histone variant macroH2A1.1, but not macroH2A1.2, leads to increased iPSC reprogramming by unclear mechanisms. Materials &amp; methods:Cleavage under targets and tagmentation (CUT&amp;Tag) allows robust epigenomic profiling of a low cell number. We performed an integrative CUT&amp;Tag-RNA-Seq analysis of macroH2A1-dependent orchestration of iPSCs reprogramming using human endothelial cells. Results:We demonstrate wider genome occupancy, predicted transcription factors binding, and gene expression regulated by macroH2A1.1 during reprogramming, compared to macroH2A1.2. MacroH2A1.1, previously associated with neurodegenerative pathologies, specifically activated ectoderm/neural processes. Conclusion:CUT&amp;Tag and RNA-Seq data integration is a powerful tool to investigate the epigenetic mechanisms occurring during cell reprogramming.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Epigenomics

ISSN

1750-1911

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

17

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

863-877

UT code for WoS article

001085184900001

EID of the result in the Scopus database

2-s2.0-85174748445