Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F23%3A00134176" target="_blank" >RIV/00216224:14110/23:00134176 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41598-023-36699-9" target="_blank" >https://www.nature.com/articles/s41598-023-36699-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-023-36699-9" target="_blank" >10.1038/s41598-023-36699-9</a>

Result language

angličtina

Original language name

Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue

Original language description

Mechanosensory ion channels are proteins that are sensitive to mechanical forces. They are found in tissues throughout the body and play an important role in bone remodeling by sensing changes in mechanical stress and transmitting signals to bone-forming cells. Orthodontic tooth movement (OTM) is a prime example of mechanically induced bone remodeling. However, the cell-specific role of the ion channels Piezo1 and Piezo2 in OTM has not been investigated yet. Here we first identify the expression of PIEZO1/2 in the dentoalveolar hard tissues. Results showed that PIEZO1 was expressed in odontoblasts, osteoblasts, and osteocytes, while PIEZO2 was localized in odontoblasts and cementoblasts. We therefore used a Piezo1(floxed/floxed) mouse model in combination with Dmp1(cre) to inactivate Piezo1 in mature osteoblasts/cementoblasts, osteocytes/cementocytes, and odontoblasts. Inactivation of Piezo1 in these cells did not affect the overall morphology of the skull but caused significant bone loss in the craniofacial skeleton. Histological analysis revealed a significantly increased number of osteoclasts in Piezo1(floxed/floxed);Dmp1(cre) mice, while osteoblasts were not affected. Despite this increased number of osteoclasts, orthodontic tooth movement was not altered in these mice. Our results suggest that despite Piezo1 being crucial for osteoclast function, it may be dispensable for mechanical sensing of bone remodeling.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10605 - Developmental biology

Project

<a href="/en/project/GA22-02794S" target="_blank" >GA22-02794S: Mechanosensing as a mechanism controlling odontogenesis across vertebrates</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

13

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

1-9

UT code for WoS article

001007856900061

EID of the result in the Scopus database

2-s2.0-85161912279