Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F24%3A00139440" target="_blank" >RIV/00216224:14110/24:00139440 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae453/7687436?login=true" target="_blank" >https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae453/7687436?login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkae453" target="_blank" >10.1093/nar/gkae453</a>

Result language
angličtina
Original language name
Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors
Original language description
Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GX21-22593X" target="_blank" >GX21-22593X: Identification and characterization of proteins involved in G-quadruplex and R-loop metabolism and their relationship with DNA replication</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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