RAD51 separation of function mutation maintenance but preserves DSB repair
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F24%3A00139744" target="_blank" >RIV/00216224:14110/24:00139744 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.isci.2024.109524" target="_blank" >https://doi.org/10.1016/j.isci.2024.109524</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.isci.2024.109524" target="_blank" >10.1016/j.isci.2024.109524</a>
Alternative languages
Result language
angličtina
Original language name
RAD51 separation of function mutation maintenance but preserves DSB repair
Original language description
Homologous recombination (HR) protects replication forks (RFs) and repairs DNA double-strand breaks (DSBs). Within HR, BRCA2 regulates RAD51 via two interaction regions: the BRC repeats to form filaments on single-stranded DNA and exon 27 (Ex27) to stabilize the filament. Here, we identified a RAD51 S181P mutant that selectively disrupted the RAD51-Ex27 association while maintaining interaction with BRC repeat and proficiently forming filaments capable of DNA binding and strand invasion. Interestingly, RAD51 S181P was defective for RF protection/restart but proficient for DSB repair. Our data suggest that Ex27-mediated stabilization of RAD51 filaments is required for the protection of RFs, while it seems dispensable for the repair of DSBs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GX21-22593X" target="_blank" >GX21-22593X: Identification and characterization of proteins involved in G-quadruplex and R-loop metabolism and their relationship with DNA replication</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
iScience
ISSN
2589-0042
e-ISSN
2589-0042
Volume of the periodical
27
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
19
Pages from-to
1-19
UT code for WoS article
001222093600001
EID of the result in the Scopus database
2-s2.0-85189497725