In vitro analysis of seven syphilis-causing Treponema pallidum strains revealed inherent growth rate differences

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F25%3A00142264" target="_blank" >RIV/00216224:14110/25:00142264 - isvavai.cz</a>

Result on the web

<a href="https://www.nature.com/articles/s41598-025-18827-9" target="_blank" >https://www.nature.com/articles/s41598-025-18827-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-18827-9" target="_blank" >10.1038/s41598-025-18827-9</a>

Result language

angličtina

Original language name

In vitro analysis of seven syphilis-causing Treponema pallidum strains revealed inherent growth rate differences

Original language description

Previous in vitro long-term cultivation studies of Treponema pallidum subsp. pallidum (T. pallidum) strains have indicated potential differences in the length of generation times among individual strains. In the present study, we have determined in vitro growth rates of seven T. pallidum strains including three from the Nichols-like cluster (DAL-1, Madras, and Haiti B) and four from the SS14-like cluster (Mexico A, SS14, Grady, and Philadelphia 1). Despite the observed considerable variability, the results of standard subcultures identified growth differences between the two clusters during the long-term cultivation. Furthermore, in vitro monocultures with defined inoculum revealed differences among individual strains. During three week-long binary co-cultivations of seven strains (n = 21), different growth rates were confirmed for individual strains (p &lt; 0.001) using PCR amplicon sequencing of genomic regions differentiating treponemal-pairs. The order of strains by decreasing growth rate in vitro was DAL-1, Madras, Mexico A, Haiti B, SS14, Grady, and Philadelphia 1. While the generation time of strain DAL-1 was 32.97 h, the slowest strain, Philadelphia 1, had generation time 43.5 h. These experiments revealed significant physiological differences between the T. pallidum strains, which may also be involved in the variable presentation of syphilis symptoms observed in previous decades.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

1-8

UT code for WoS article

001582550500042

EID of the result in the Scopus database

2-s2.0-105017417099