n immunocytochemical analysis of growing axons in a silicone chamber prefilled with artificial sponge matrix

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F96%3A00024506" target="_blank" >RIV/00216224:14110/96:00024506 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

n immunocytochemical analysis of growing axons in a silicone chamber prefilled with artificial sponge matrix

Original language description

We have used antibodies against growth associated protein (GAP-43), phosphorylated neurofilament protein of 200 kDa molecular weight (RT-97) and substance P (SP) to analyze regrowing axons and their features in a silicone chamber filled with resorbable sponge matrix within the first two weeks after sciatic nerve transection in the rat. Growing axons identified with the GAP-43 antibody extended over a distance of about 7 mm from the proximal stump at 7 days and grew over a 10 mm gap within 14 days. Thisis a markedly longer distance than in the case of the standard chamber model without artificial sponge matrix. The regrowing axons were labelled with RT-97 already on the 7th day up to a distance of 5 mm and they made up about 75% of all axons in the first segments. The number of RT-97-positive axons did not increase significantly over the next 7 days, although they could be identified over a longer distance. Some of the growing axons expressed SP-like immunoreactivity (LI) 14 days, but

Czech name

Imunohistochemická analýza rostoucích axonů v silikonové cisterně

Czech description

Rychlost reinervace tubulizovaného nervu byla studována imunohistochemickou detekcí GAP43 a RT97.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

<a href="/en/project/GA309%2F93%2F0134" target="_blank" >GA309/93/0134: Migration and Differentiation of Schwann Cells in the Adult Organism</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

1996

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Histochemica

ISSN

0065-1281

e-ISSN

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Volume of the periodical

98

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

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UT code for WoS article

A1996UK55300002

EID of the result in the Scopus database

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