Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14160%2F20%3A00117760" target="_blank" >RIV/00216224:14160/20:00117760 - isvavai.cz</a>

Alternative codes found

RIV/68407700:21460/20:00347868

Result on the web

<a href="http://www.prolekare.cz/casopisy/ceska-slovenska-farmacie/2020-1-24/dvojite-obalene-pelety-se-semipermeabilnim-obalem-z-ethylcelulosy-urcene-k-detekci-inhibitoru-cholinesteras-122472" target="_blank" >http://www.prolekare.cz/casopisy/ceska-slovenska-farmacie/2020-1-24/dvojite-obalene-pelety-se-semipermeabilnim-obalem-z-ethylcelulosy-urcene-k-detekci-inhibitoru-cholinesteras-122472</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors

Original language description

Currently, nerve agents are often used in terrorist attacks or assassinations. In such cases, it is necessary to detect them quickly, accurately and easily right in the field. Detection tubes, which are small devices containing pellets with immobilized cholinesterase and detection reagents, meet these conditions. Their detection mechanism is based on a highly sensitive enzymatic Ellman reaction, when in the absence of cholinesterase inhibitors the pellets develop a visible yellow color, whereas in their presence the carriers retain the original color. The rate of reaction, its sensitivity and the distinct color transition are the key points of the research. In this experiment, double-coated pellets were prepared. The first coating contained the butyrylcholinesterase immobilized in hypromellose, while the second coating consisted of ethylcellulose and triethyl citrate. Based on the properties of such carriers, samples containing lactose dispersed in the ethylcellulose coating were also prepared, which was expected to have an effect on increasing the permeability of the coating and hence the detection rate and color intensity. In addition to selected physicochemical properties, carriers were evaluated for enzyme activity, sensitivity and color transition intensity. Samples showing the best properties were subjected to a 24-months stability test at three different temperatures and humidity.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Česká a slovenská farmacie

ISSN

1210-7816

e-ISSN

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Volume of the periodical

69

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

24-32

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85085539451