Crystal structure of a cytokinic-glucoside-specific beta-glucosidase from Zea mays

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00002764" target="_blank" >RIV/00216224:14310/00:00002764 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Crystal structure of a cytokinic-glucoside-specific beta-glucosidase from Zea mays

Original language description

Zm-p60.1, a cytokinin-glucoside-specific b-glucosidase from maize, is a key enzyme involved in plant development and growth. It has been over-expressed in soluble form from Escherichia coli with a His tag at its N-terminus, the recombinant protein has been purified and crystallized at room temperature using PEG 4000 as main precipitant. A flash-annealed monoclinic crystal diffracted to high resolution (beyond 2 A) with space group P21, and unit-cell parameters a = 55.66 A, b = 110.72 A, c = 72.94 A, b =92.10 deg. Resulting crystal structure was solved by molecular replacement.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/VS96096" target="_blank" >VS96096: Laboratory of Molecular Plant Physiology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologické listy

ISSN

0366-0486

e-ISSN

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Volume of the periodical

65

Issue of the periodical within the volume

3-4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

3

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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