Monitoring of Dithiothreitol Clearance by Micellar Electrokinetic Chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00004409" target="_blank" >RIV/00216224:14310/01:00004409 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Monitoring of Dithiothreitol Clearance by Micellar Electrokinetic Chromatography
Original language description
Dithiothreitol (DTT) is a well known sulfhydryl reagent used to protect the sulfhydryl groups of small molecular compounds and proteins in a variety of biochemical applications. The radioprotective action of the compound has received significant attention for a number of years. Recently DTT has been applied in studies on heat cell killing as a result of interest in hyperthermia as a cancer treatment modality. DTT is also routinely used to solubilize recombinant proteins from inclusion bodies. Previous methods used to determine DTT in solutions have included spectrophotometry and HPLC with UV spectrophotometric and fluorescence detection. We proposed the application of the on-column reaction of DTT with the reactive disulphide - 2,2-dipyridyldisulphide(DPDS) for monitoring of DTT clearance. DPDS and a sample of DTT are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the DPDS a
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA525%2F00%2F0785" target="_blank" >GA525/00/0785: Acidification processes in sulphide wastes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Book of abstract 2nd International symposium "Separations in BioSciences SBS 2001"
ISBN
80-7080-437-8
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
86
Publisher name
Czech Chemical Society
Place of publication
Prague
Event location
Prague
Event date
Jan 1, 2001
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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