Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00004521" target="_blank" >RIV/00216224:14310/01:00004521 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry

Original language description

Metallothionein (MT) from rabbit liver was analysed by differential pulse polarography (DPP with DME), cyclic voltammetry (CV), square wave voltammetry (SWV), and chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE) under various conditions. The highest sensitivity of the MT determination was obtained with CPSA which produced a well developed peak H due to catalytic hydrogen evolution at highly negative potentials. The highest peak H was obtained in borate buffer atpH 7.6. In this medium subnanomolar concentrations of MT were detectable. In the adsorptive transfer stripping (medium exchange) experiments determination of few femtomoles of MT in 5 ml aliquots of the analyte was possible. CPSA determination of MT in various tissues of carp (Cyprinus cyprio) yielded values in agreement with the published data.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

<a href="/en/project/GV204%2F97%2FK084" target="_blank" >GV204/97/K084: Electrodes modified with nucleic acids and proteins.New tools in biochemical and biomedical research.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Anal. Chem.

ISSN

0003-2700

e-ISSN

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Volume of the periodical

2001

Issue of the periodical within the volume

20

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

4801

UT code for WoS article

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EID of the result in the Scopus database

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