Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00005395" target="_blank" >RIV/00216224:14310/01:00005395 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic
Original language description
Species Staphylococcus aureus belongs to clinically important gram-positive cocci. Some S. aureus strains produce TSST-1 toxin, which causes human disease called Toxic Shock Syndrom (TSS). Two forms of TSS were differentiated: the first type occurs in connection with menses, the second one is formed of cases, when TSS is a complication of another staphylococcal disease, for exampte food poisoning. Data about abiGty of S. aureus strains to produce TSST-1 toxin are clearly different. Therefore, we analyzed genomic DNAs of 26 S. aureus strains isolated from patients in the Czech Republic in connection with TSS. Staphylococcal isolates were analyzed by PCR using specific oligonucleotide primers to confirm presence of specific sequences of tst gene. To determine genetic variability, we performed macrorestriction analysis using PFGE, which enables determination of 20 different PFGE types. Strains were divided into 8 PFGE groups (ET1-ET8) according to Smal restriction patteros based on the si
Czech name
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Czech description
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Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
V. Pracovní setkání biochemiků a molekulárních biologů
ISBN
80-210-2538-7
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
25
Publisher name
Masarykova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Jan 1, 2001
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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