Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00005468" target="_blank" >RIV/00216224:14310/02:00005468 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography

Original language description

Micellar electrokinetic capillary chromatography (MEKC) has been developed as a promising method for the determination of lignans in plant samples. The separation conditions have been optimised with respect to the different parameters including SDS and acetonitrile concentration, pH of the background electrolyte, separation voltage and temperature of capillary. The background electrolyte consisting of 40 mM SDS and 35 % acetonitrile in 10 mM tetraborate buffer (pH 9.3) was found to be the most suitableelectrolyte for this analysis. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 25 oC gave the best separation of lignans. The inter-day reproducibility of the peak areas and the migration times were below 2.0 %. The results of MEKC analyses were compared with those obtained by capillary electrochromatography (CEC) and reversed-phase high performance liquid chromatography (RP-HPLC). The possibilities of using this method for determination of lignans in dr

Czech name

Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography

Czech description

Micellar electrokinetic capillary chromatography (MEKC) has been developed as a promising method for the determination of lignans in plant samples. The separation conditions have been optimised with respect to the different parameters including SDS and acetonitrile concentration, pH of the background electrolyte, separation voltage and temperature of capillary. The background electrolyte consisting of 40 mM SDS and 35 % acetonitrile in 10 mM tetraborate buffer (pH 9.3) was found to be the most suitableelectrolyte for this analysis. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 25 oC gave the best separation of lignans. The inter-day reproducibility of the peak areas and the migration times were below 2.0 %. The results of MEKC analyses were compared with those obtained by capillary electrochromatography (CEC) and reversed-phase high performance liquid chromatography (RP-HPLC). The possibilities of using this method for determination of lignans in dr

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA303%2F00%2FD062" target="_blank" >GA303/00/D062: Bioavailability and mechanism of antioxidant activity of phenolic acids and lignans</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

253

UT code for WoS article

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EID of the result in the Scopus database

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