Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00005543" target="_blank" >RIV/00216224:14310/02:00005543 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography

Original language description

A new method for specific determination of dithiothreitol (DTT) using micellar electrokinetic chromatography and on-column reaction with reactive disulfide - 2,2-dipyridyldisulfide is described. DTT is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of DTT is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 2.5 mM (correlation coefficient 0.993) with a detection limit of 2.5 mM. The inter-day reproducibility of thepeak area was 1.35 % and the inter-day reproducibility of the migration time 0.56 %. The method can be applied for DTT monitoring both in chemical and biological systems.

Czech name

Monitoring of dithiothreitol clearance by means of micellar electrokinetic chromatography

Czech description

A new method for specific determination of dithiothreitol (DTT) using micellar electrokinetic chromatography and on-column reaction with reactive disulfide - 2,2-dipyridyldisulfide is described. DTT is in this reaction quantitatively transformed into a mixed disulfide concomitantly with formation of equimolar amount of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of DTT is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 2.5 mM (correlation coefficient 0.993) with a detection limit of 2.5 mM. The inter-day reproducibility of thepeak area was 1.35 % and the inter-day reproducibility of the migration time 0.56 %. The method can be applied for DTT monitoring both in chemical and biological systems.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA525%2F00%2F0785" target="_blank" >GA525/00/0785: Acidification processes in sulphide wastes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography B

ISSN

0378-4347

e-ISSN

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Volume of the periodical

770

Issue of the periodical within the volume

1-2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

237

UT code for WoS article

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EID of the result in the Scopus database

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