Analysis of membrane proteome of Paracoccus denitrificans by two-dimensional electrophoresis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00006413" target="_blank" >RIV/00216224:14310/02:00006413 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Analysis of membrane proteome of Paracoccus denitrificans by two-dimensional electrophoresis

Original language description

In facultative anaerobic bacterium P. denitrificans, a broad range of substrates can serve as carbon sources and terminal electrone acceptors. Successfull adaptation of this bacterium to altered conditions depends on expression of metabolically active proteins. For example, nitrate induces expression of some denitrification enzymes. Many important redox proteins are membrane-associated. For comprehensive study on changes of proteins level in this bacterium, we have optimized a method using two-dimensional electrophoresis (2-DE). Our work represents the first analysis of P. denitrificans proteome using 2-DE. Membrane suspension of anaerobically grown cells of Paracoccus denitrificans was prepared by combination of enzymatic and osmotic lysis, centrifugation and ulgtracentrifugation. We have tested various detergents and procedures for membrane proteins presolubilization (e.g., SDS, CHAPS, dodecyl-â-D-maltoside with 6-aminocaproic acid). Sample buffer and IEF gel composition effects on 2

Czech name

Analysis of membrane proteome of Paracoccus denitrificans by two-dimensional electrophoresis

Czech description

In facultative anaerobic bacterium P. denitrificans, a broad range of substrates can serve as carbon sources and terminal electrone acceptors. Successfull adaptation of this bacterium to altered conditions depends on expression of metabolically active proteins. For example, nitrate induces expression of some denitrification enzymes. Many important redox proteins are membrane-associated. For comprehensive study on changes of proteins level in this bacterium, we have optimized a method using two-dimensional electrophoresis (2-DE). Our work represents the first analysis of P. denitrificans proteome using 2-DE. Membrane suspension of anaerobically grown cells of Paracoccus denitrificans was prepared by combination of enzymatic and osmotic lysis, centrifugation and ulgtracentrifugation. We have tested various detergents and procedures for membrane proteins presolubilization (e.g., SDS, CHAPS, dodecyl-â-D-maltoside with 6-aminocaproic acid). Sample buffer and IEF gel composition effects on 2

Type

D - Article in proceedings

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA203%2F01%2F1589" target="_blank" >GA203/01/1589: Mechanisms involved in regulation of respiratory systems in denitrification bacteria by environmental factors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

XVIII. biochemický zjazd. Zborník.

ISBN

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ISSN

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e-ISSN

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Number of pages

1

Pages from-to

160

Publisher name

Slovenská spoločnosť pre biochémiu a molekulárnu biológiu pri SAV

Place of publication

Stará Lesná

Event location

Vysoké Tatry, Stará Lesná 10.-13.9.2002

Event date

Jan 1, 2002

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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