Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00006912" target="_blank" >RIV/00216224:14310/02:00006912 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Identification of Salmonella enterica serovar Typhimurium genes associated with growth suppression in stationary-phase nutrient broth cultures and in the chicken intestine

Original language description

Over 2,800 Tn5 mutants of Salmonella enterica serovar Typhimurium have been screened for the loss of ability to suppress the multiplication of a spectinomycin-resistant but otherwise isogenic S. Typhimurium strain, when this was added to 24 hour LB brothcultures of the mutants. Selected growth non-suppressive (GNS) mutants were defective in respiration (insertions in arcA and fnr), amino acid biosynthesis (aroA and aroD), nutrient uptake and its regulation (tdcC and crp), and in chemotaxis (fliD). In the last GNS mutant, the transposon inactivated yhjH, an ORF with unknown function which shows homology to di-guanylate cyclase and novel two-component signal transduction proteins. In newly-hatched chickens, all the mutants, with the exception of the fliD mutant, were also unable to suppress colonisation of the alimentary tract by the parent strain inoculated one day later. Defined mutations in luxS or sdiA, genes which contribute to quorum sensing in S. Typhimurium, had no effect on the

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/LN00A016" target="_blank" >LN00A016: BIOMOLECULAR CENTER</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Archives of Microbiology

ISSN

0302-8933

e-ISSN

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Volume of the periodical

178

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

411

UT code for WoS article

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EID of the result in the Scopus database

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