CKI1, a putative sensor histidine kinase is essential for proper female gametophyte formation in Arabidopsis thaliana.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010681" target="_blank" >RIV/00216224:14310/04:00010681 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

CKI1, a putative sensor histidine kinase is essential for proper female gametophyte formation in Arabidopsis thaliana.

Original language description

Embryo sac formation is a fundamental step in plant sexual reproduction. However, the key players driving female gametophyte development remain elusive. Employing reverse genetics, we have identified CKI1, a putative sensor histidine kinase, as a key regulatory component during female gametophyte formation in Arabidopsis thaliana. Using PCR-based screen we have identified a line carrying insertion of the autonomous transposable element En-1 in the coding region of CKI1 gene. Distorted genotype ratios and distribution of the insertional mutant allele cki1-i in the progeny of the identified mutant plant suggested lowered viability and/or function of the cki1-i gametes. In accordance to that, the results of reciprocal back-crosses have shown that cki1-i cannot be transmitted through the female germ line. Confocal laser scanning microscopy (CLSM) analysis of developing embryo sacs from cki1-i/CKI1 pistils did not reveal any deviations from the wild type phenotype in the first four developm

Czech name

CKI1, predpokladana receptorova histidinkinaza je nezbytna pro normalni vyvoj samiciho gametofytu u Arabidopsis thaliana.

Czech description

Tvorba embryonalniho vaku je nezbytnou soucasti pohlavni reprodukce rostlin. Presto zustavaji zakladni faktory ridici tvorbu samiciho gametofytu nezname. Za pouziti reverzni genetiky jsme identifikovali CKI1, predpokladanou receptorovou histidinkinazu jako klicovou regulacni komponentu behem tvorby samiciho gametofytu u Arabidopsis thaliana. Pomoci PCR vyhledavani jsme identifikovali lini nesouci inzerci kukuricneho autonomniho elementu En-1 v kodujici oblasti genu CKI1. Zmena v segregacnich pomerech adistribuce inzercni mutantni alely cki1-i v potomstvu identifikovane mutantni rostliny nazancovaly snizenou zivotnost, resp. funkcnost gamet nesoucich mutantni alelu cki1-i. Ve shode s tim, vysledky zpetnych reciprokych krizeni potvrdily, ze alela cki1-inemuze byt do dalsiho potomstva prenesena prostrednictvim samici zarodecne linie. Analyza embryonalnich vaku u rostlin cki1-i/CKI1 pomoci laserove konfokalni mikroskopie (CLSM), ukazala normalni vyvoj vsech embryonalnich vaku do stadia F

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Boook of Abstracts, Cytokinematics 2004, Microscopy of Live Cells in the Post Genomics Era. The 8th Symposium on Light Microscopy & Live Cells in Hradec Kralove

ISBN

80-86225-60-7

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

22

Publisher name

RNDr. F. Skopec, CSc.-NUCLEUS HK

Place of publication

Hradec Králové

Event location

Hradec Králové

Event date

Jan 1, 2003

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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