Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010788" target="_blank" >RIV/00216224:14310/04:00010788 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana.

Original language description

One of the possible approaches to a plant gene function study in model plant Arabidopsis thaliana leads across T-DNA mutagenesis. Collection of 2500 T-DNA lines have been obtained by A. thaliana transformation with Agrobacterium tumefaciens containing plasmid pPCVRN4 with HPT selectable gene (Koncz et al. 1994). Screening of individual lines revealed three different mutations with defects in immature seed development (marked 1265, 1293, 1321) which have been characterised and analysed in detail. One T-DNA insert was determined in each line and the mutations were confirmed to be monogenic and recessive-lethal by genetic analysis. Morphological studies of embryo development were performed by clearing in chloral hydrate. Mutant embryos were blocked in certain steps in the process necessary for embryo viability and development. In 1265 mutation defect in globular stage was observed. It was associated with abnormal pattern of cell division connected with shoot apical meristem formation duri

Czech name

Identifikace a characterizace tří genů determinujících embryogenezi prostřednictvím T-DNA mutací u Arabidopsis thaliana.

Czech description

Jedním z možných přístupů studia funkcí rostlinných genů u Arabidopsis thaliana je T-DNA mutageneze. Kolekce 2500 T-DNA linií po transformaci Agrobacterium tumefaciens byla využita při vyhledávání mutací s embryonálními defekty. Tři tyto mutace (1265, 1293, 1321) podrobně analyzovány a charakterizovány. Genetická analýza potvrdila monogenní a recesivní charakter těchto mutací.

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Abstract Book of the 15th International Conference on Arabidopsis Research, July 11-14, Berlin, Germany.

ISBN

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ISSN

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e-ISSN

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Number of pages

1

Pages from-to

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Publisher name

Max Planck Institute of Molecular Plant Physiology

Place of publication

Berlin, Germany

Event location

Berlin, Germany

Event date

Jul 11, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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