Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011235" target="_blank" >RIV/00216224:14310/04:00011235 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode
Original language description
The group cysteine-containing peptides (metallothioneines, phytochelatins, and glutathione) plays an important role in active homeostasis and detoxification of metals in animal, plant, and/or microorganism cells. In this study we describe a novel approach to electroanalysis of cys-containing peptides and proteins. This approach is based on catalytic processes (presodium wave, peak H, Brdicka current) proceeding at negative potentials (ca. 1.8 V, Ag/AgCl/3M KCl) on the hanging mercury drop electrode (HMDE). A presodium wave is current-potential response accompanied with catalytic hydrogen evolution from aqueous solutions in the presence of proteins. It was found that the sulfhydryl(SH) groups in proteins are responsible for presodium catalytic processes. The highest sensitivity in the determination was observed with derivative chronopotentiometric stripping analysis (CPSA), which monitored the catalytic signal known as peak H. The determination of the low content of Cys-peptides was suc
Czech name
Elektrochemické stanovení biomolekul obsahujících cystein pomocí katalytických reakcí na rtuové elektrod
Czech description
Elektrochemické stanovení biomolekul obsahujících cystein pomocí katalytických reakcí na rtuové elektrod.

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Book of Abstracts - 55th Annual Meeting of the ISE
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
431-431
Publisher name
International Society of Electrochemistry
Place of publication
Thessaloniki, Greece
Event location
Thessaloniki, Greece
Event date
Feb 19, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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