The protonation/deprotonation equilibrium of thymidine and azidothymidine studied by UV spectra and multivariate curve resolution

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011238" target="_blank" >RIV/00216224:14310/04:00011238 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The protonation/deprotonation equilibrium of thymidine and azidothymidine studied by UV spectra and multivariate curve resolution

Original language description

The aim of our work was to study of protonation/deprotonation equilibrium of AZT and AZU in aqueous solutions with different concentrations of NaCl by using UV spectra connected with chemometrics. Calibration and correct function of pH meter (electrode)was performed by acido-basic potenciometric titration (HCl and NaOH), of which parameters were calculated by means of GranLs method. Correct way how to determine ionization constants was verified using thymidine and uridine for which the pKa value was known. The absorption spectra were measured from 240 to 320 nm at temperature 25C and various ionic strength from 0.05M to 0.25M. The absorption maxima of thymidine and AZT are very close and have values of 267 and 266.5 nm for pH 8, respectively. The wavelength of maxima of uridine and AZU were identical 262nm, but the absorbance of AZU has hypochromical shift. Firstly, from non-smoothed spectra the system of matrices (É, A.U., pH) was made (Fig. a), that were the input data for Multivari

Czech name

Protonaní/deprotonaní rovnováhy thymidinu a azidothymidinu studované pomocí UV spektrometrie a chemometrie

Czech description

Usilím naší práce bylo získat pK hodnoty u thymidinu a azidothymidinu z vodných roztoků změřených pomocí UV spektroskopie.K vyhodnocování spekter thymidinu a azidothymidinu bylo použito chemometrické metody; Multivariate Curve Resolution MCR.

Type

D - Article in proceedings

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

sborn&iacute;k- XLVII Zjazd PTchem i SITPChem Wroclaw 2004

ISBN

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ISSN

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e-ISSN

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Number of pages

1

Pages from-to

1232

Publisher name

Polskie Towarystwo Chemiczne

Place of publication

Wroclaw, Polsko,2004

Event location

Wroclaw, Polsko

Event date

Sep 12, 2004

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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