Reconstruction of Mycobacterial Protein Rv2579 in Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00025489" target="_blank" >RIV/00216224:14310/04:00025489 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Reconstruction of Mycobacterial Protein Rv2579 in Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26
Original language description
Gene rv2579 was found in complete genome sequence of Mycobacterium tuberculosis H37Rv and was supposed to code haloalkane dehalogenase due to 68% sequence identity with LinB which is haloalkane dehalogenase from Sphingomonas paucimobilis UT26 [1]. Objective of the study was to test membership of Rv2579 protein within haloalkane dehalogenase family. The homology model of protein Rv2579 was compared with the crystal structure of haloalkane dehalogenase LinB [2]. This analysis revealed that 6 out of 19 amino acid residues which form an active site and entrance tunnel are different in LinB and Rv2579. The 6 different amino acids were cumulatively mutated in LinB. Final six-fold mutant was presumed to have active site and entrance tunnel of Rv2579 and exhibited dehalogenase activity with the haloalkanes tested, confirming that Rv2579 is a member of the haloalkane dehalogenase family [3]. Consequently the rv2579 gene was cloned from Mycobacterium bovis 5032/66 into Escherichia coli. Rv2579 p
Czech name
Rekonstrukce mykobakterialního proteinu Rv2579 v haloalkán dehalogezáze Lin B z bakterie Sphingomonas paucimobilis UT26
Czech description
přednáška Marty Monincove na 6th International Conference on Protein Stabilization

Project
<a href="/en/project/LN00A016" target="_blank" >LN00A016: BIOMOLECULAR CENTER</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
6th International Conference on Protein Stabilization
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
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Publisher name
Slovak Society of Chemical Engineering
Place of publication
Bratislava, Slovakia
Event location
Bratislava
Event date
Sep 26, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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