High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00013567" target="_blank" >RIV/00216224:14310/05:00013567 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/05:00345103
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches
Original language description
PA-IIL is a fucose-binding lectin from Pseudomonas aeruginosa that is closely related to the virulence factors of the bacterium. Previous structural studies have revealed a new carbohydrate-binding mode with direct involvement of two calcium ions (Mitchell E, Houles C, Sudakevitz D, Wimmerova M, Gautier C, Perez S,Wu AM, Gilboa-Garber N Imberty A. Structural basis for selective recognition of oligosaccharides from cystic fibrosis patients by the lectin PA-IIL of Pseudomonas aeruginosa. Nat Struct Biol 2002;9:918921). A combination of thermodynamic, structural, and computational methods has been used to study the basis of the high affinity for the monosaccharide ligand. A titration microcalorimetry study indicated that the high affinity is enthalpy driven. The crystal structure of the tetrameric PA-IIL in complex with fucose and calcium was refined to 1.0 Ĺ resolution and, in combination with modeling, allowed a proposal to be made for the hydrogen-bond network in the binding site. Calc
Czech name
Vysoká afinita vazby fukosy u lektinu Pseudomonas aeruginosa lectin PA-IIL: krystalová struktura komplexu s vysokým rozlišením 1.0 A v kombinaci s termodynamickými metodami a přístupy výpočetní chemie
Czech description
Vysoká afinita vazby fukosy u lektinu Pseudomonas aeruginosa lectin PA-IIL: krystalová struktura komplexu s vysokým rozlišením 1.0 A v kombinaci s termodynamickými metodami a přístupy výpočetní chemie
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proteins: Structure, Function, and Bioinformatics
ISSN
0887-3585
e-ISSN
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Volume of the periodical
58
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
735-746
UT code for WoS article
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EID of the result in the Scopus database
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