Quartz crystal biosensor for detection of sugars and glycated hemoglobin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00013672" target="_blank" >RIV/00216224:14310/05:00013672 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Quartz crystal biosensor for detection of sugars and glycated hemoglobin
Original language description
3-Aminophenylboronic acid (APBA) was used for construction of affinity mass sensors for determination of saccharides and glycated hemoglobin using complexation reaction with diol groups. Two approaches were tested for the bioligand layer fabrication-incorporation of APBA inside a thicker matrix and immobilization as a self-assembled monolayer, respectively. The direct affinity sensor with APBA embeded in the structure of glutaraldehyde-crosslinked bovine serum albumine provided linear response to mono-and dissacharides in the range from 0.1 to 15.0 mg/ml. This biosensor was also used for kinetic analysis of the interaction of boronic acid with diols; the values of association and dissociation constants were determined. The sensors with a monolayer ofboronic groups were better suited for binding of glycated hemoglobin, probably due to improved steric access to the ligand.
Czech name
Biosensor pro detekci sacharidů a glykovaného hemoglobinu na bázi křemenných krystalů
Czech description
3-Aminofenylboritá kyselina (APBA) byla použita jako ligand imobilizovaný na povrchu piezoelektrického afinitního sensoru, který sloužil pro stanovení sacharidů (glukosa, fruktosa, sorbitol, aj.) a glykovaného hemoglobinu (marker u diabetiků). Byly porovnány různé typy imobilizačních postupů, poskytujících buď monovrstvu nebo matrix s navázanou APBA.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
530
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
10
Pages from-to
75-84
UT code for WoS article
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EID of the result in the Scopus database
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