GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014595" target="_blank" >RIV/00216224:14310/05:00014595 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes
Original language description
Azaarenes contain nitrogen atom in place of a carbon atom. The majority of the azaarenes found in the environment originates from anthropogenic sources. Concentrations of NPAHs found in the environment are reported to be one to two orders of magnitude lower than PAH concentrations, but their biological effects can be of similar magnitude. Very few studies on the genotoxicity of azaarenes are available in the literature. In the present study, a preliminary profile of both the toxic and genotoxic potential of 5 PAHs and their 20 aza-analogues was investigated. To assess the toxic and genotoxic activity a GPF assay based on the yeast Saccharomyces cerevisiae was selected. To compare the sensitivity of this eukaryotic short-term assay with bacterial screening tests, Toxi-Chromotest for toxicity and SOS-Chromotest for genotoxicity assessment were also performed. This comparison indicates that in most cases the yeast GFP assay is apparently of comparable specificity to the bacterial toxicity
Czech name
GFP screeningový model pro detekci toxické a genotoxické aktivity azaarenů
Czech description
Genotoxicita azaarenů

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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