Activation of Ah-receptor by humic substances

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014613" target="_blank" >RIV/00216224:14310/05:00014613 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Activation of Ah-receptor by humic substances
Original language description
Humic substances (HS) are ubiquitous in the environment. However, some studies indicate that HS can induce a direct adverse effect on human health and hormone-like effects on fish, amphibians and invertebrates. In the present study we investigated possible biochemical mechanism of HS toxicity using reporter gene in vitro bioassay with H4IIE.luc cells. H4IIE.luc are stably transfected hepatoma cells with a luciferase reporter gene that is activated via cytosolic aryl hydrocarbon receptor (AhR). The mostpotent activator of AhR is 2,3,7,8-tetrachlorodibenzo-p-dioxin, but number of structurally diverse compounds has also been found to activate AhR. Aim of this study was to evaluate the dioxin-like activity of humic substances standards purchased from IHSSand Sigma-Aldrich companies. HS were dissolved in 0.05M NaOH, subsequently, lipophilic compounds (including fragments of HS) were extracted by liquid-liquid extraction with hexane/dichloromethane. Organic extract of HS was further treate
Czech name
Aktivace AhR huminovými látkami
Czech description
Přeložte si anglický.

Project
<a href="/en/project/GP525%2F05%2FP160" target="_blank" >GP525/05/P160: In vitro models for study of endocrine disruption - effects of traditional and new types of persistent organic pollutants</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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