Change of the Protein p53 Electrochemical Signal According to its Structural Form - Quick and Sensitive Distinguishing of Native, Denatured, and Aggregated Form of the "Guardian of the Genome"

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00015697" target="_blank" >RIV/00216224:14310/06:00015697 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/06:00103383 RIV/00216208:11130/06:471

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Change of the Protein p53 Electrochemical Signal According to its Structural Form - Quick and Sensitive Distinguishing of Native, Denatured, and Aggregated Form of the "Guardian of the Genome"

Original language description

Presence of mutated and/or structurally modi.ed (e.g., denatured, aggregated) protein p53 form is associated with several disorders such as Alzheimers disease, Parkinsons disease, prion diseases, and many types of tumours. The aim of this work was to distinguish native, denatured and aggregated form of full-length p53 by .ow injection analysis coupled with electrochemical detector (FIA-ED). Firstly FIA-ED method used for protein native form determination was optimized (detection limit 45.8 amol per 5 llinjection; 3_S/N). In addition the technique was applied to identify p53 structural forms (denatured and aggregated). It was found out that denatured form provides about three times higher electrochemical response (protein structure unfolding, approachof more electroactive centers - aminoacid residues - towards electrode surface) in comparison with native form. On the other hand, aggregated form o.ers lower response (steric eclipse of electroactive protein parts) when compared with the

Czech name

Změna elektrochemického signálu proteinu P53 podle jeho strukturní formy - rychlé a citlivé rozlišení nativní, denaturované a agregované formy -Strážce genomu.

Czech description

Změna elektrochemického signálu proteinu P53 podle jeho strukturní formy - rychlé a citlivé rozlišení nativní, denaturované a agregované formy -Strážce genomu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

<a href="/en/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Study of protection mechanisms for stress-induced by heavy metals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

The Protein Journal

ISSN

1572-3887

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

23-32

UT code for WoS article

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EID of the result in the Scopus database

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