Development of a combined setup for simultaneous detection of total and glycated haemoglobin content in blood samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00016801" target="_blank" >RIV/00216224:14310/06:00016801 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Development of a combined setup for simultaneous detection of total and glycated haemoglobin content in blood samples
Original language description
An original setup for analysis of glycated haemoglobin (HbA,) in blood is reported. The construction employed a combination of the piezoelectric biosensor for glycated haemoglobin and the flow-through photometric sensor for total haemoglobin (Hb). The modification of gold electrodes with 3-aminophenylboronic acid (APBA) as a specific ligand was studied; the chemisorbed conjugate of APBA with a long-chain thiocompound provided the best affinity for Hb(A1c). The effect of various operating parameters, such as flow rate and instrumental setup, was optimised. The total haemoglobin content was analysed as absorbance of the haemoglobin-cyanide derivative at 540 nm. Only one standard (calibrator) diluted in various ratio was necessary for calibration and 1 mul of blood was sufficient for analysis. The full range of Hb(A1c) content (4-15%) in blood can be analysed; the working ranges of total and glycated haemoglobin were 50-2000 and 10-90 mu g/ml, respectively. The developed method was succe
Czech name
Vývoj kombinovaného systému pro současné stanovení celkového a glykovaného hemoglobinu ve vzorcích krve
Czech description
Byl vyvinut kombinovaný systém na bázi piezoelektrického biosensoru s imobilizovanou kyselinou aminofenylboritou pro specifickou detekci glykvaného hemoglobinu, paralelně se spektrofotometricky stanovuje celkový hemoglobin. Systém je spojen s automatickýprůtočným analyzátorem FIAlab a je vhodný pro měření vzorků krve.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biosensors & Bioelectronics
ISSN
0956-5663
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
1952-1959
UT code for WoS article
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EID of the result in the Scopus database
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