Measurement of Long Range 1H-19F Scalar Coupling Constants and their Glycosidic Torsion Dependence in 5-Fluoropyrimidine Substituted RNA.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00016826" target="_blank" >RIV/00216224:14310/06:00016826 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Measurement of Long Range 1H-19F Scalar Coupling Constants and their Glycosidic Torsion Dependence in 5-Fluoropyrimidine Substituted RNA.

Original language description

Long range scalar 5J(H1',F) couplings were observed in 5-fluoropyrimidine substituted RNA. We developed a novel S3E-19F-alfa/beta-edited NOESY experiment for quantitation of these long range scalar 5J(H1',F), where the J-couplings can be extracted from inspection of intraresidual (H1',H6) NOE crosspeaks. The 5J(H1',F) couplings can be used to restrict the glycosidic torsion angle which defines the orientation of the base with respect to the ribose sugar moiety. Quantum chemical calculations were exploited to investigate the relation between scalar couplings and conformations around the glycosidic bond in oligonucleotides. The theoretical dependence of the observed 5J(H1',F) couplings on the torsion angle Ó can be described by a generalized Karplus relationship. The corresponding density functional theory (DFT) analysis is outlined.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of the American Chemical Society

ISSN

0002-7863

e-ISSN

—

Volume of the periodical

128

Issue of the periodical within the volume

17

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—