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High-throughput characterization of enzymes from genomic and proteomic projects-multivariate statistical approach

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00017056" target="_blank" >RIV/00216224:14310/06:00017056 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    High-throughput characterization of enzymes from genomic and proteomic projects-multivariate statistical approach

  • Original language description

    Hight throughput genomic and proteomic methods release a lot of novel enzymes every year which require systematic characterization and cataloguing of their properties. Here, we illustrate a novel approach, using multivariate statistics to characterize and describe a protein family with broad substrate specificity. Principle of the approach consists in multivariate statistic method, principle component analysis [1], which enabled firstly to choose sufficient set of 30 substrates from 194 halogenated compounds respecting maximum variability in physical-chemical properties [2]. Quick and reliable enzymatic assay follows the selection and produces an activity data of particular proteins with selected substrates. Third step is application of principal component analysis on enzyme activity data matrix to describe the difference in substrate specifities. In paralell, kinetic constants Km and kcat were measured.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů