Spectrophotometric analysis of nucleic acid bases in binary and ternary mixtures by partial least squares and artificial neural networks

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00019330" target="_blank" >RIV/00216224:14310/06:00019330 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Spectrophotometric analysis of nucleic acid bases in binary and ternary mixtures by partial least squares and artificial neural networks
Original language description
The contribution shows a comparative study of the use of partial least squares (PLS) and artificial neural networks (ANNs) to analyze nucleic acid bases (adenine - A, cytosine - C, thymine -T) in mixtures by UV-Vis spectrophotometry. Multivariate calibration based on a suitable experimental design (ED) and soft modeling for the quantitative analysis of fully overlapped ultraviolet spectra of A+C binary and/or A+C+T ternary systems were employed. The optimal ANN architecture, enabling to model the system, was established by means of TRAJAN program. Using the multivariate statistical method SIMCA the linearity of the PLS model for dilute solutions containing two or three bases in acetate buffer (pH 4.7) was proved. A combination of the chemometric methods and derivative spectrophotometry was applied. The ability of ANN and PLS to model binary and ternary systems was evaluated on the basis of the root means square error for prediction (RMSEP) and the agreement factor (AF). For the determi
Czech name
Spektrofotometrická analýza bazí nukleových kyselin v binární a ternární směsi pomocí PLS a ANN
Czech description
Spektrofotometrická analýza bazí nukleových kyselin v binární a ternární směsi pomocí PLS a ANN

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
3rd International Symposium on Computer Applications and Chemometrics in Analytical Chemistry SCAC 2006
ISBN
963-9696-01-3
ISSN
—
e-ISSN
—
Number of pages
1
Pages from-to
7-7
Publisher name
University of Veszprém
Place of publication
Veszprém
Event location
Lake Balaton, Hungary
Event date
Jul 1, 2006
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
—

Similar results(10)