Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00020262" target="_blank" >RIV/00216224:14310/07:00020262 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/07:00108108

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions

Original language description

Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of carboxyl ester bonds. Their connection with development has made them a suitable marker of development in plants. In the present work, we focused on the fluorimetric determination of the plant esterases in plant cell cultures (tobacco BY-2 cells and early somatic embryos of Norway spruce, clone 2/32) with respect to application the method for the study of programmed cell death and the influence of cadmium(II) ions on the plant cells. The programmed cell death has been triggered by sodium nitroprusside and glucose oxidase. The determination of the esterase activity by the proposed technique in a cell extract determined very small difference in enzymeactivity, which was a reliable marker of metabolic changes. In addition, the esterase activity of spruce somatic embryos decreased with the increase in medium Cd concentration.

Czech name

Aplikace fluorimetrické analýzy rostlinných esteráz při studiu progamované buněčné smrti a vliv kademnatých iontů

Czech description

Aplikace fluorimetrické analýzy rostlinných esteráz při studiu progamované buněčné smrti a vliv kademnatých iontů

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Study of protection mechanisms for stress-induced by heavy metals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologica Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

551-555

UT code for WoS article

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EID of the result in the Scopus database

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