Genome rearrangements in host-range mutants of the plyvalent staphylococcal bacteriophage 812

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00020477" target="_blank" >RIV/00216224:14310/07:00020477 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Genome rearrangements in host-range mutants of the plyvalent staphylococcal bacteriophage 812

Original language description

Mutations extended the host range of the polyvalent bacteriophage 812 of the family Myoviridae in up to 95 % of Staphylococcus aureus strains and 43 % of strains of different coagulase-positive and coagulase-negative Staphylococcus species. Mutational changes in the genome of several previously isolated host-range mutants of phage 812 were identified and described. Host-range mutant 812F1 harbors a deletion in endolysin gene that arose together with intron excision. Mutants 812i, 812b, 812p and 812F3 harbor deletion in the structural gene orf8 that results from a genome rearrangement that is associated with intron insertion. This rearrangement was also detected in the genome of the closely related phages, U16 and phi131. Another intron was discovered in the recA812 gene in mutants 812i, 812b, 812p and 812F3. An insertion was found in a non-coding region of the restriction fragment PstI-O of mutants 812b, 812F3 and 812g and phages U16 and phi131. Results of this study contribute to the

Czech name

Genomické změny u mutant polyvalentního fága 812 v rozmezí hostitele

Czech description

Mutations extended the host range of the polyvalent bacteriophage 812 of the family Myoviridae in up to 95 % of Staphylococcus aureus strains and 43 % of strains of different coagulase-positive and coagulase-negative Staphylococcus species. Mutational changes in the genome of several previously isolated host-range mutants of phage 812 were identified and described. Host-range mutant 812F1 harbors a deletion in endolysin gene that arose together with intron excision. Mutants 812i, 812b, 812p and 812F3 harbor deletion in the structural gene orf8 that results from a genome rearrangement that is associated with intron insertion. This rearrangement was also detected in the genome of the closely related phages, U16 and phi131. Another intron was discovered in the recA812 gene in mutants 812i, 812b, 812p and 812F3. An insertion was found in a non-coding region of the restriction fragment PstI-O of mutants 812b, 812F3 and 812g and phages U16 and phi131. Results of this study contribute to the

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA203%2F03%2F0515" target="_blank" >GA203/03/0515: Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

52

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

331-338

UT code for WoS article

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EID of the result in the Scopus database

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